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Western blot analysis Total proteins were extracted from pancreatic or cells in RIPA lysis buffer (Applygen, C1055) containing a 1% protease inhibitor cocktail and 1 mM PMSF

Patients with heart failure with reduced ejection fraction experienced a 35% reduction in major cardiovascular events (hazard ratio 0.65, 95% CI 0.49-0.87), while those with heart failure with preserved ejection fraction saw a 31% reduction (hazard ratio 0.69, 95% CI 0.51-0.91)

A cross-disease meta-GWAS identifies four new susceptibility loci shared between systemic sclerosis and Crohns disease